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| Aragão,Annelize Zambon Barbosa; Teocchi,Marcelo Ananias; Fregolente,Maria Clara Duarte; Gatti,Maria Silvia Viccari; Pires,Alexandre Vaz; Yano,Tomomasa. |
| Twenty seven (48.2%) culture supernatants of 56 Escherichia coli isolated from diarrheic lamb feces (7 to 10 days old) in São Paulo State, Brazil, presented positive results to suckling mice assay (fluid accumulation) but none caused cytopathic effects on Vero and CHO cells, indicating that these strains did not produced LT or VT toxins. PCR assays showed that these 27 E. coli strains harbored estA, that codifies for STa, but not for stx1, stx2 or cnf genes. The positive STa strains were checked for genes that codify for F41, F17 and K99 fimbriae, wich are considered colonization factors in ETEC. Only F17 was detect in two samples (7.4%). Twelve of 27 STa positive carried hlyA gene and presented hemolytic activity in blood Agar. Presence of rotavirus was... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Lambs; STa; Colibacillosis; Escherichia coli. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000500015 |
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| Bezerra,W.G.A.; Horn,R.V.; Silva,I.N.G.; Siqueira,R.A.S.; Lucena,R.B.; Havt,A.; Medeiros,P.H.Q.S.; Maciel,W.C.. |
| ABSTRACT This study reports a co-infection of Escherichia coli and Salmonella in a free-living ruddy ground dove (Columbina talpacoti) received at the Laboratory of Ornithological Studies of the State University of Ceará, Brazil. The bird presented diarrhea, leg paralysis and anorexia, and died shortly after. Necropsy was then performed and samples from lung, kidney, liver and intestine were collected for microbiological and histopathological analyses. Escherichia coli was isolated from cloacal swab, lung and kidney samples. Salmonella ser. Saintpaul was identified in liver and spleen samples. Escherichia coli isolates were tested for the presence of eight diagnostic genes for diarrheagenic pathotypes (STEC, ETEC, EPEC, EIEC, EAEC) with conventional... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Colibacillosis; Salmonellosis; Antimicrobial Resistance. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501236 |
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| Oliveira,ES; Cardozo,MV; Borzi,MM; Borges,CA; Guastalli,EAL; Ávila,FA. |
| ABSTRACT Avian pathogenic Escherichiacoli (APEC) virulence mechanism has been continuously studied and it is believed to be multifactorial and because of this, this work aimed to characterize potentially APEC strains isolated from free-range hens. Isolates were submitted to PCR for the detection of virulence genes, which were of high prevalence. In vivo inoculation of day-old chicks revealed that 49 of these strains were of high and intermediate pathogenicity. In addition, isolates were submitted to antimicrobials susceptibility test with the majority of the strains presenting multiresistance. Phylogenetic analysis showed a greater presence of potentially APEC isolates in-group B2. In addition, high heterogeneity was detected among the isolates byXbaI... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: APEC; Backyard chickens; Colibacillosis; Pathogenicity; Virulence factors. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100326 |
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| Santos,A.S.; Lima,D.C.V.; Fernandes,E.F.T.S.; Albuquerque,P.P.F.; Gouveia,G.V.; Sá,M.C.A.; Costa,M.M.; Pinheiro Júnior,J.W.; Mota,R.A.. |
| ABSTRACT Enteric diseases of bacterial origin are frequent in the pig industry, of particular notoriety are the colibacillosis that mainly affect piglets and cause great damage to the swine industry worldwide. The aim of the study was to analyze phylogenetics, to detect biofilm production, and to determine antimicrobial resistance profile in 126 strains of Escherichia coli isolated from swabs obtained from fragments of the small intestines of 235 healthy pigs killed in slaughterhouses in Pernambuco (Brazil) using polymerase chain reaction (PCR), adherence to microplates test and disc diffusion technique. Of the analyzed samples, 88.10% (111/126) were classified in phylogenetic group B1; 4.76% (6/126) in group D; 3.97% (5/126) in group B2 and, 3.17% (4/126)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pig industry; Colibacillosis; Phylogeny; Antibiogram; Multi-resistance. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401309 |
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| Aguilar-Urquizo,E; Itza-Ortiz,MF; Sangines-Garcia,JR; Pineiro-Vázquez,AT; Reyes-Ramirez,A; Pinacho-Santana,B. |
| ABSTRACT Natural antimicrobials, known as phytobiotics, are used in bacterial infections. The objective of this study was to evaluatethe phytobiotic activity, in vitro and in vivo, of an extract and an essential oil of Piper auritum and Ocimum basilicum on avian Eschericia coli serotype O2 in broiler chickens experimentally infected. For the in vitro test, extracts at 4, 8, 12 and 16% in water-based solvent or alcohol at 70% were prepared from leaves of both plants. In the essential oils, solvents at 10% were used. A concentration of 1×108 CFU mL-1 of bacteria was seeded and on each sense disc, 25 µL of the extract or essential oil were poured, except on the positive or negative control. The diameter of the inhibition zone (DIZ) of bacterial growth was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Colibacillosis; Phytobiotic; Piper auritum; Ocimum basilicum. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100327 |
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| Knöbl,Terezinha; Gomes,Tânia Aparecida Tardelli; Vieira,Mônica Aparecida Midolli; Ferreira,Fernando; Bottino,José Américo; Ferreira,Antônio José Piantino. |
| Three hundred and fifty strains of E. coli isolated from septicemic poultry from seven states of Brazil were examined for presence of nine adhesion-encoding genes, hemagglutination and adherence to chicken tracheal cells (in vitro). Analysis of the strains by colony hybridization tests demonstrated that 93.7% of the isolates were fim +, 17% pap+ and 5.7% were sfa+. The mannose sensitive fimbriae occur with similar frequency in APEC isolated from all Brazilians states, while significant differences among pap and sfa genes distributions were observed. The results showed that 0.85% and 0.28% of APEC were positive for genes that encoded enteroaggregative adhesins and EPEC adherence factor, respectively. None of APEC was positive for DA, afa, Bfp and Eae... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: APEC; Escherichia coli; Adhesins; Colibacillosis; Poultry. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300033 |
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| Costa,M.M.; Drescher,G.; Maboni,F; Weber,S.S.; Schrank,A.; Vainstein,M.H.; Schrank,I.S.; Vargas,A.C.. |
| Virulence factors and antimicrobial resistance patterns of Escherichia coli isolates were evaluated. A total of 80 E. coli isolates were evaluated, being 64 from clinical samples (intestinal content and fragments of organs from diarrheic piglets), seven from feces of clinically healthy piglets and sows, and nine environmental samples (five from facilities, two from feed, one from insect, and one from waste). Molecular characterization was performed by PCR detection of fimbriae and toxin genes and plasmid content determination. The isolates were also characterized according to their resistance or sensitivity to the following drugs: ampicillin, trimethoprim:sulfamethoxazole, tetracycline, amikacine, colistin, norfloxacin, florfenicol, enrofloxacin,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Swine; Colibacillosis; Fimbriae; Toxin; Antimicrobial resistance. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000100004 |
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| Pereira,Daniele Araujo; Vidotto,Marilda Carlos; Nascimento,Karla Alvarenga; Santos,Anne Caroline Ramos dos; Mechler,Marina Lopes; Oliveira,Luís Guilherme de. |
| ABSTRACT: Enterotoxigenic Escherichia coli (ETEC) is the major cause of diarrhea in newborn and weaned pigs. Bacteria adhesion to the host cell is considered a specific phenomenon among fimbrial and non-fimbrial adhesins with their respective receptors on enterocytes. Enteric disorders are related with the fimbriae F4 (K88), F5 (K99), F6 (987P), F41, and F18. In addition to ETEC, another category of E. coli , porcine pathogenic E. coli (PEPEC),can cause diarrhea in pigs; it produces the porcine attaching and effacing-associated (Paa) adhesin in, which is capable to cause a typical lesion known as an attaching and effacing (A/E) lesion. Immunization of sows with adhesin is important to stimulate the production of antibodies and their subsequent transfer... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Colibacillosis; Diarrhea; Vaccines; Swine; Colostrum.. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000801430 |
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